A petition on the Brisbane City Council website is calling for council to include protected bi-directional bike lanes in the Edward Street renewal project.
The proposed bike lanes would run through Edward Street.
They would start at Ann Street near Central Train Station and end at the Brisbane City Botanical Gardens.
Donald Campbell, the principal petitioner and co-convener of the Brisbane CBD Bicycle User Group (CBD BUG), says now is the perfect opportunity to demand action.
"If they don't integrate protected bike lanes in the Edward Street renewal project, then it will cost more money to come back and do it in the future," Mr Campbell said.
"CBD roads are intimidating for cyclists and perceived safety is a crucial factor.
"Sydney has started the process of protected bike lanes and has had an 100% increase in cycling."
According to Mr Campbell, bi-directional lanes would be the most cost-efficient model.
"The way it works is that one traffic lane would be removed from traffic usage and there would be no loss of footpath space," he said.
"That traffic lane is mostly used for car parking.
"It would be a two way lane, separated from the road and footpath by concrete protection."
The impact on businesses: minimal or extreme?
Mr Campbell said that the proposed lanes would have minimal impact on businesses.
However, Eckersley's store manager, Selina Binns, does not agree.
"Parking is difficult in the city and the lack of parking spaces has impacted our store quite severely already," Ms Binns said.
"These lanes would definitely have an impact."
While Mr Campbell said that most people who go to businesses in the CBD do so via public transport, Ms Binns believes they travel to Eckersley's via car.
"People don't want to be carrying art supplies and canvases on trains and buses," she said.
"They don't want to be carrying them around the city all day."
Antique Guild head of operations, Joshua O'Donnell, said it is hard to determine how customers arrive at their store.
"Many of our customers arrive on foot but as to whether they are staying at the hotel next door, or have found a park elsewhere in the city, or have caught public transport, it's all very hard to say," Mr. O'Donnell said.
However, he said the proposed bike lanes may impact the store's operational side.
"Sometimes we have big shipments of furniture delivered to our store, so we utilise the loading zone outside of the old port office," he said.
"Depending on which side of the road the bike lanes are proposed for, they may or may not have an impact on our operational side of business."
Bicycle Queensland development officer, Andrew Demack, said the organisation agrees with the proposed bike lanes.
Mr Denmark said it will have a positive impact on businesses and pedestrians.
"A CBD that is better for bike riders and pedestrians will be better for retail and other businesses because consumers will have more choice in how they travel to their destinations," he said.
"More travel choice leads to consumers being able to choose their shopping outlets based on quality and service rather than simply what's easy to drive to."
Mr Denmark said that if the benefits of improving safety for bike riders are made clear to Brisbane residents, then there is no reason for a backlash if the petition is approved.
"Making it easier to ride to CBD destinations is a big challenge for Brisbane City Council in making Brisbane a truly bike-friendly city," he said.
"We can't claim Brisbane as a bike-friendly city until all those who want to ride to work are able to do so in safety."
The likelihood of approval?
Last year, Mr Campbell submitted a petition requesting protected bike lanes be placed on Sylvan Road in Toowong.
Despite receiving 755 signatures, it was not approved by council.
"Car parks have been placed above people getting maimed or killed," Mr Campbell said.
Mr Campbell said if the current petition (which has 91 signatures) is not approved then the Brisbane CBD BUG will continue campaigning for cyclists.
"We will be campaigning actively and we will not give up," he said.
"Edward Street is begging for protected bike lanes."
Brisbane City Council declined to comment on the issue.
